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Prescribed practical B1 - Making slides

A slide is a thin piece of glass used to hold objects which are examined under a microscope.

Most slides will already be made up for you. If you do get the chance to prepare your own slide, here鈥檚 what to do:

Plant cells

  1. Peel a thin, transparent layer of cells from the inside of an onion.
  2. Place cells on a microscope slide.
  3. Add a drop of water or iodine (a chemical stain).
  4. Lower a coverslip onto the onion cells using forceps or a mounted needle. This needs to be done gently to prevent trapping air bubbles.

Animal cells

  1. Remove cells from the inside of your cheek using a cotton bud.
  2. Smear the cotton bud onto a microscope slide.
  3. Add a drop of methylene blue (a chemical stain).
  4. Lower a onto the cheek cells using forceps or a mounted needle. This needs to be done gently to prevent trapping air bubbles.

Chemical stains are used to make some cell parts more obvious.

Image gallerySkip image gallerySlide 1 of 2, Making slides with animal cells, Animal cells Cheek cells

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