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Carrying out a genetic cross

Monohybrid crosses

Genetic crosses of single gene combinations (monohybrid inheritance) can be shown and examined using . This shows the possible offspring combinations could be produced, and the of these combinations can be calculated.

Worked example 1

The height of pea plants is controlled by a single gene which has two alleles: tall and short.

The tall allele is dominant and is shown as T.

The small allele is recessive and is shown as t.

Draw out an empty Punnett square, like the one below, to help predict the possible gene combinations.

TT
t
t
t
T
T
t
T
T

The female is TT - tall

The male is tt - short

The Punnett square below shows the possible allele combinations of the offspring produced when two homozygous pea plants, tall female and short male, are bred.

TT
tTtTt
tTtTt
t
TTt
TTt
t
TTt
TTt

Adding the combinations into each box shows that all the possible offspring have the same allele combination - Tt.

This means that all the offspring produced will be tall.

Worked example 2

In this genetic cross, the mother is heterozygous for the wet earwax gene (Ee). The father is homozygous for the dry earwax allele (ee).

Ee
eEeee
eEeee
e
EEe
eee
e
EEe
eee

Half of the possible offspring will have wet earwax (Ee). The other half will have dry earwax (ee).

These examples are single gene combinations, but remember that most phenotypes are controlled by multiple genes.