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Sassuolo 1-0 Juventus: Gregoire Defrel fires second-half winner
Gregoire Defrel scored a second-half winner as Sassuolo dented Juventus' chances of European qualification in Serie A.
Defrel reacted fastest to an attempted clearance by the visitors before pivoting and firing past Mattia Perin.
Perin was standing in for regular goalkeeper Wojciech Szczesny, who reported chest pains in midweek.
The defeat leaves Juve, who were docked 15 points for illicit transfer activity in January, outside the European spots.
Massimiliano Allegri's side are seventh in the Serie A table, four points behind sixth-placed Atalanta, who have played one game fewer.
Victory for Sassuolo moves them up to 11th.
Meanwhile, Roma beat Udinese 3-0 to stretch their winning run in the league to three games.
Edoardo Bove opened the scoring in the first half before Lorenzo Pellegrini doubled their advantage after the break.
Englishman Tammy Abraham came off the bench to add a third in injury time to ensure Jose Mourinho's side remain third in the table.